Балл 1.72

Число соавторов публикации - 5
Целевой показатель госзадания:

  • Научные публикации в журналах, индексируемых в WEB of Science - Формула: (3+ИФ)/корень квадратный из Nавторов = (3+0.855)/корень квадратный из 5 (2.2361) = 1.72
  • Научные публикации в журналах, индексируемых в SCOPUS - Формула: (2+ИФ)/корень квадратный из Nавторов = (2+0.302)/корень квадратный из 5 (2.2361) = 1.03

Запись создана: 12-08-2018 11:18
Последнее изменение: 26-09-2018 15:49

Phylogenetic Diversity of the Sulfur Cycle Bacteria in the Bottom Sediments of the Chersonesus Bay

Тип публикации

Статья в журнале

Перевод
Оригинальная статья:
Филогенетическое разнообразие бактерий цикла серы в донных осадках Херсонесской бухты // Микробиология. 2018. Т. 87, № 3. С. 279–290
Язык eng
Журнал Microbiology

ISSN: 0026-2617 ; Онлайн ISSN: 1608-3237
Год 2018
Выходные данные Том: 87
Номер: 3
Страницы: 372–381
Авторы
  1. Bryukhanov A. L.
    Faculty of Biology Lomonosov Moscow State University, Moscow, Russia (ru)
    Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia (ru)
  2. Vlasova M. A.
    Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia (ru)
  3. Malahova T. V.
  4. Perevalova A. A.
    Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia (ru)
  5. Pimenov N. V.
    Winogradsky Institute of Microbiology, Research Center of Biotechnology Russian Academy of Sciences, Moscow, Russia (ru)
DOI 10.1134/S0026261718030025
Усл. п. л.
Даты Поступила в редакцию: 19.09.2017
Опубликована онлайн: 02.06.2018
Абстракт The Black Sea is the largest meromictic basin, in the bottom sediments of which a powerful biogenic process of sulfide production occurs. The goal of the present work was to obtain data on phylogenetic diversity of the sulfur cycle microorganisms (sulfate-reducing and sulfur-oxidizing bacteria) in the Black Sea coastal gas-saturated bottom sediments. The samples were collected in the Chersonesus (Blue) Bay near Sevastopol from whitish bacterial mats of sulfurettes, and from the upper layer of the nearby seabed. Using DNA isolated from the native samples and obtained enrichment cultures, PCR analysis was performed with oligonucleotide primers specific to the fragments of the 16S rRNA genes of the main subgroups of sulfatereducing bacteria (SRB) and to the fragments of the dsrB gene (both reductive and oxidative types), encoding the β-subunit of dissimilatory (bi)sulfite reductase, the key enzyme in the sulfur cycle, inherent in both sulfate- reducing and sulfur-oxidizing microorganisms. The presence of 16S rRNA gene fragments specific to the genera Desulfobacterium, Desulfobacter, Desulfococcus–Desulfonema–Desulfosarcina, and Desulfovibrio–Desulfomicrobium was detected in the DNA samples isolated from coastal bottom bacterial mats. Usage of denaturing gradient gel electrophoresis (DGGE) with subsequent sequencing of reamplified dsrB gene fragments revealed that according to deduced amino acid sequences encoded by the dsrB gene (reductive type), SRB from the coastal gas-saturated bottom sediments of the Black Sea had the highest homology (92−99%) with the dsrB gene of cultured SRB belonging to the genera Desulfovibrio, Desulfatitalea, Desulfobacter, and Desulfobacterium, as well as with uncultured SRB strains from various marine habitats, such as bottom sediments of the Northern and Japanese seas. Deduced amino acid sequences encoded by the oxidative dsrB gene had the highest homology (90−99%) with the relevant sequences of the genera Thiocapsa, Thiobaca, Thioflavicoccus, and Thiorhodococcus.
Ключевые слова: sulfate-reducing bacteria, sulfur-oxidizing (thionic) bacteria, the Black Sea, bottom sediments, microbial mats, microbial communities, dissimilatory (bi)sulfite reductase, dsrB gene
SCOPUS WoS Гос. задание

РИНЦ
Статус Нет

SCOPUS
Статус Да
Квартиль Q3
Рейтиг 0.302 (2017)
Ссылка Открыть в SCOPUS
WoS
Статус Да
Квартиль Q4
Импакт-фактор 0.855 (2017)
Ссылка Открыть в WoS
ВАК
Статус Нет

Публикация отнесена к гос.заданию:
0828-2018-0001 (АААА-А18-118020890090-2) «Молисмологические и биогеохимические основы гомеостаза морских экосистем»

Тэги